Waters Hplc System Cleaning Procedure

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Waters hplc system cleaning procedure.

Responsibility quality control officer executive. Label bottles correctly with bottle content and filling date expiry date. Use solvent inlet filters to protect the system from incoming particles. Reverse osmosis to remove most contaminants b.

Residues or contaminations may block filters or capillaries. To clean the system. To clean the system. Place a b c and d seal wash and needle wash lines into a clean.

Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307. Technical assistant 3 2 checking. Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa. Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.

To clearly lay down the procedure for cleaning. Ion exchange to remove any remaining ions. No injections are needed with that. Maintaining your hplc uhplc uplc systems.

Remove all solvent filters. Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data. When working with any lc system setting it up and storing it is very important. Use only hplc grade solvents and water filtered through 0 2.

Controlling contamination in lc ms and hplc ms systems 715001307. Cleaning decontamination columns general guidelines flow cells general. Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307. 2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.

When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines. 3 0 responsibility 3 1 doing. Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems. For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.

Disconnect the column and attach a union and flow directly to waste. It is necessary to restore the system flow path to a near new level of cleanliness note. Water flush to clear customer s mobile phases from the system. The purification process should include all of the following steps.